ZFNs (Zinc Finger Nucleases)

References/Resources: https://www.patreon.com/the_Crux

All videos on Genetic Engineering: https://www.youtube.com/playlist?list=PL0Ymnd-zt4Ij9qp5ziU0TkyF42LUcgHQf

What are zinc finger nucleases or ZFNs? How are ZFNs used for genetic/genome engineering and gene editing? How were these programmable nucleases engineered by combining zinc finger DNA-binding proteins with the FokI restriction enzyme cleavage or nuclease domain? In this video, I discuss the mechanistic basis of ZFNs, beginning with the structure and DNA recognition properties of C2H2 zinc finger proteins and the dimerization-dependent cleavage mechanism of the FokI nuclease. The video explains how arrays of zinc fingers can be engineered to target extended DNA sequences for genome editing. Finally, we cover the major limitations of ZFNs.

00:00 Reagents
00:36 General idea
01:39 Zinc finger proteins
04:36 FokI Nuclease
07:03 ZFNs
10:11 Engineering
11:11 ZFN issues
12:23 Alternatives

CRISPR-Cas9 in genome editing: https://youtu.be/7ESZTE6rjLI

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*Must watch playlists*
All videos on Genetic Engineering: https://www.youtube.com/playlist?list=PL0Ymnd-zt4Ij9qp5ziU0TkyF42LUcgHQf
All videos on DNA Replication: https://youtube.com/playlist?list=PL0Ymnd-zt4IgLefDaeqicJbCBnbLqnDJ7
All videos on Transcription: https://www.youtube.com/playlist?list=PL0Ymnd-zt4Ij2VcAOHNUweftSElVsc-MX
All videos on Translation: https://www.youtube.com/playlist?list=PL0Ymnd-zt4IjAAAMlPLDIXbs692Yp14xa

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