TALENs (Transcription Activator-Like Effector Nucleases)

References/Resources: https://www.patreon.com/the_Crux

All videos on Genetic Engineering: https://www.youtube.com/playlist?list=PL0Ymnd-zt4Ij9qp5ziU0TkyF42LUcgHQf

What are TALENs (Transcription activator-like effector nucleases) and how are they used in gene editing? How do TALEs function and bind target DNA, and can be used for genome editing and genome engineering? How to TAL-effectors function? How do TALENs and ZFN compare? In this video, I discuss the mechanistic and functional basis of TALENs, their origins, and specific DNA recognition code of the Repeat Variable Diresidue (RVD). I also discuss how the TALEN arrays are engineered for gene editing and genome engineering. Finally, we compare TALENs with other nucleases used in genome engineering.

00:00 General idea
01:04 TALEs
03:58 RVD and DNA binding
06:59 TALEN mechanism
08:36 5’ Thymine significance
10:48 Cons

ZFNs in genome editing: https://youtu.be/u2ALvgt4NYA
CRISPR-Cas9 in genome editing: https://youtu.be/7ESZTE6rjLI

References/Resources: https://www.patreon.com/the_Crux

*Must watch playlists*
All videos on Genetic Engineering: https://www.youtube.com/playlist?list=PL0Ymnd-zt4Ij9qp5ziU0TkyF42LUcgHQf
All videos on DNA Replication: https://youtube.com/playlist?list=PL0Ymnd-zt4IgLefDaeqicJbCBnbLqnDJ7
All videos on Transcription: https://www.youtube.com/playlist?list=PL0Ymnd-zt4Ij2VcAOHNUweftSElVsc-MX
All videos on Translation: https://www.youtube.com/playlist?list=PL0Ymnd-zt4IjAAAMlPLDIXbs692Yp14xa

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