RNA Expression Vectors – Design, Applications, and Examples (In vitro Transcription)

References/Resources: https://www.patreon.com/the_Crux

All videos on Genetic Engineering: https://www.youtube.com/playlist?list=PL0Ymnd-zt4Ij9qp5ziU0TkyF42LUcgHQf

RNA Expression vectors (different from protein expression vectors) are used for the sole purpose of making RNA from a template DNA. In this video, we discuss some of the common applications of RNA expression vectors and their rules/principles of design. Finally, we look at two examples, including in vitro transcription to understand how design principles and applications change our design of RNA expression vectors.

Jump to your favorite section:
00:00 RNA Expression Vector
01:00 Application
02:00 Considerations
04:35 Promoters and Terminators
06:33 Example 1
08:43 In vitro Transcription
11:45 Example 2

References/Resources: https://www.patreon.com/the_Crux

Suggested videos:
Restriction Enzyme Cloning: https://youtu.be/j4aSfi8MaXA
Bacterial Transcription Termination: https://youtu.be/yiii0Pef_6k
Operons (lac, ara, trp): https://www.youtube.com/playlist?list=PL0Ymnd-zt4Ij4jwm3B8TG7tXeby6MQYaR

*Must watch playlists*
All videos on Genetic Engineering: https://www.youtube.com/playlist?list=PL0Ymnd-zt4Ij9qp5ziU0TkyF42LUcgHQf
All videos on DNA Replication: https://youtube.com/playlist?list=PL0Ymnd-zt4IgLefDaeqicJbCBnbLqnDJ7
All videos on Transcription: https://www.youtube.com/playlist?list=PL0Ymnd-zt4Ij2VcAOHNUweftSElVsc-MX
All videos on Translation: https://www.youtube.com/playlist?list=PL0Ymnd-zt4IjAAAMlPLDIXbs692Yp14xa

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