Protein Expression Vectors – Expression Host and Recombinant Proteins [Part 1]

References/Resources: https://www.patreon.com/the_Crux

All videos on Genetic Engineering: https://www.youtube.com/playlist?list=PL0Ymnd-zt4Ij9qp5ziU0TkyF42LUcgHQf

Protein Expression vectors are used to make protein from the template DNA. In this video, we discuss some of the common applications of protein expression vectors and the complications involved with protein expression when changing the host i.e. heterologous protein expression. The applications are typically functional studies or protein purification and production based. To understand protein expression vector biology, we compare prokaryotic and eukaryotic expression systems, especially the host physiology. There are different types of proteins and depending on the complexity of protein, the protein expression approach has to be modified – I use the example of membrane trafficking and disulfide bonds. Finally, we explore the molecular components for a protein expression vector and the extra engineering (tags and epitopes) that goes with it.

Jump to your favorite section:
00:00 Goals
00:36 Application
01:45 Expectation
03:03 Host Comparison
05:30 Host Limitations
07:27 Vector Design
08:30 Engineering

References/Resources: https://www.patreon.com/the_Crux

Suggested video:
RNA Expression Vectors: https://youtu.be/4-43spjrmrE

*Must watch playlists*
All videos on Genetic Engineering: https://www.youtube.com/playlist?list=PL0Ymnd-zt4Ij9qp5ziU0TkyF42LUcgHQf
All videos on DNA Replication: https://youtube.com/playlist?list=PL0Ymnd-zt4IgLefDaeqicJbCBnbLqnDJ7
All videos on Transcription: https://www.youtube.com/playlist?list=PL0Ymnd-zt4Ij2VcAOHNUweftSElVsc-MX
All videos on Translation: https://www.youtube.com/playlist?list=PL0Ymnd-zt4IjAAAMlPLDIXbs692Yp14xa

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