Plasmid and Phagemid cloning vector – Selectable vs. Screening marker

References/Resources: https://www.patreon.com/the_Crux

This video discusses the general biology of cloning vectors focusing on plasmids and phagemids. We discuss how plasmids replicate, their general properties, diversity, origins, and many other important details. This will also help you understand the evolution of cloning vectors from a standard Plasmid to Phagemid. This video also provides you with a conceptual framework of selectable and screening markers, discussed with examples.

Jump to your favorite section:
00:00 Outline
00:59 Natural plasmids
04:26 Plasmid size
06:26 ColE1 plasmid replication
10:40 pBR322 plasmid
12:36 pUC18/19 plasmid
14:23 Phagemid (pBlueScript)
17:26 Selectable markers
20:08 Screening markers
23:32 Blue-White screening

Patreon Page: https://www.patreon.com/the_Crux

Suggested videos:
(M13 RCA) Rolling Circle Amplification: https://youtu.be/ae2Mg7USvUQ
Mitochondrial DNA Replication: https://youtu.be/THZFH9l1SeQ
Lac Operon: https://youtu.be/ajFLReouZrU

*Must watch playlists*
All videos on Genetic Engineering: https://www.youtube.com/playlist?list=PL0Ymnd-zt4Ij9qp5ziU0TkyF42LUcgHQf
All videos on DNA Replication: https://youtube.com/playlist?list=PL0Ymnd-zt4IgLefDaeqicJbCBnbLqnDJ7
All videos on Transcription: https://www.youtube.com/playlist?list=PL0Ymnd-zt4Ij2VcAOHNUweftSElVsc-MX
All videos on Translation: https://www.youtube.com/playlist?list=PL0Ymnd-zt4IjAAAMlPLDIXbs692Yp14xa

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