F plasmid, BACs, Fosmid cloning vector – Fosmids are small BACs (Bacterial Artificial Chromosomes)

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This video discusses the general biology of F plasmids, Bacterial Artificial Chromosomes, BACs, and Fosmids. We start the origin of high capacity cloning vectors with the F plasmids and the development of mini F vectors. This was the inspiration for BACs which went through a few iterations involving retrofits and screening markers. Fosmids were developed at the same time as BACs, eventually leading to the conclusion that Fosmids are just BACs with smaller insert sizes that behave like cosmids.

Jump to your favorite section:
00:00 F plasmids
02:45 mini F cloning vector
05:20 BACs
06:58 Fosmids
10:16 Fosmids are small BACs

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Suggested videos:
Cosmids: https://youtu.be/SHE0lpMiitA
pUC plasmid + Blue White screening: https://youtu.be/2pAM-FTFI2c

*Must watch playlists*
All videos on Genetic Engineering: https://www.youtube.com/playlist?list=PL0Ymnd-zt4Ij9qp5ziU0TkyF42LUcgHQf
All videos on DNA Replication: https://youtube.com/playlist?list=PL0Ymnd-zt4IgLefDaeqicJbCBnbLqnDJ7
All videos on Transcription: https://www.youtube.com/playlist?list=PL0Ymnd-zt4Ij2VcAOHNUweftSElVsc-MX
All videos on Translation: https://www.youtube.com/playlist?list=PL0Ymnd-zt4IjAAAMlPLDIXbs692Yp14xa

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