CRISPRi and CRISPRa using dCas9 | CRISPR-ON and CRISPR-OFF | Beyond Cas9 gene editing

References/Resources: https://www.patreon.com/the_Crux

All videos on Genetic Engineering: https://www.youtube.com/playlist?list=PL0Ymnd-zt4Ij9qp5ziU0TkyF42LUcgHQf

What is CRISPRi (CRISPR interference)? What is CRISPRa (CRISPR activation)? What is a dCas9 and how to use dCas9 for CRISPRi and CRISPRa experiments? dCas9 can be fused with effector domains to regulate gene expression for functional studies. In addition, you can extend the CRISPRi/CRISPRa based tools to build permanent epigenetic modifications using CRISPR-ON and CRISPR-OFF. In this video, I discuss the details of dCas9 based genetic manipulations (and multiple iterations/generations of these tools) used to repress and activate genes and go beyond genome editing using CRISPR/Cas systems.

00:00 dCas9
00:52 CRISPRi (Gen 1)
02:21 CRISPRa (Gen 1)
03:36 CRISPRa (Gen 2)
06:38 Challenges
07:41 CRISPRi/a (Gen 3)
10:32 CRISPR-ON
11:38 CRISPR-OFF

CRISPR-Cas9 in genome editing: https://youtu.be/7ESZTE6rjLI
Epigenetic Replication: https://youtu.be/HB4IAiYqdzA

References/Resources: https://www.patreon.com/the_Crux

*Must watch playlists*
All videos on Genetic Engineering: https://www.youtube.com/playlist?list=PL0Ymnd-zt4Ij9qp5ziU0TkyF42LUcgHQf
All videos on DNA Replication: https://youtube.com/playlist?list=PL0Ymnd-zt4IgLefDaeqicJbCBnbLqnDJ7
All videos on Transcription: https://www.youtube.com/playlist?list=PL0Ymnd-zt4Ij2VcAOHNUweftSElVsc-MX
All videos on Translation: https://www.youtube.com/playlist?list=PL0Ymnd-zt4IjAAAMlPLDIXbs692Yp14xa

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