Base editing with CRISPR-Cas9

References/Resources: https://www.patreon.com/the_Crux

All videos on Genetic Engineering: https://www.youtube.com/playlist?list=PL0Ymnd-zt4Ij9qp5ziU0TkyF42LUcgHQf

What is Base editing? What is nCas9? How is Base editing done using CRISPR Cas9? What are the types of base editing methods? How is deamination involved in base editing? How can base editing be used? What are the limitations of base editing? In this video, I discuss the development and details of nCas9, and how nCas9 is used in base editing. There are two primary types of base editing: Adenosine base editing and Cytidine base editing. Each base editing system has its specific deaminase enzyme. Even though base editing holds great potential, it comes with some serious limitations.

00:00 nCas9
01:49 Base editing
02:36 Cytosine Base editing
06:10 Adenine Base editing
08:06 Applications
09:06 Limitations

CRISPR-Cas9 in genome editing: https://youtu.be/7ESZTE6rjLI
CRISPRi/a (dCas9): https://youtu.be/bRlQcIAtThk

References/Resources: https://www.patreon.com/the_Crux

*Must watch playlists*
All videos on Genetic Engineering: https://www.youtube.com/playlist?list=PL0Ymnd-zt4Ij9qp5ziU0TkyF42LUcgHQf
All videos on DNA Replication: https://youtube.com/playlist?list=PL0Ymnd-zt4IgLefDaeqicJbCBnbLqnDJ7
All videos on Transcription: https://www.youtube.com/playlist?list=PL0Ymnd-zt4Ij2VcAOHNUweftSElVsc-MX
All videos on Translation: https://www.youtube.com/playlist?list=PL0Ymnd-zt4IjAAAMlPLDIXbs692Yp14xa

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